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KMID : 0370220090530010034
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Yakhak Hoeji
2009 Volume.53 No. 1 p.34 ~ p.40
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Neutral and Amino Sugars Composition of a Lectin from Maackia fauriei
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Na Kwang-Heum
Park Byung-Tae
Park Jae-Wan
Han Kyong-Jin
Park Hyun-Joo
Kim Ha-Hyung
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Abstract
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The glycosylation of therapeutic glycoproteins can affect their efficacy, stability, solubility, and half-life. Analyzing the composition of monosaccharides, such as that of neutral and amino sugars, is the first step for elucidating the structure of glycan attached to glycoproteins. In the present study, neutral and amino sugars of lectin obtained from Maackia faurieiwere analyzed using an enzyme-linked lectinsorbent assay (ELLA) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Peroxidase-labeled lectins such as concanavalin A, Ricinus communis agglutinin, and soybean agglutinin were used for ELLA, since they specifically bind to the monosaccharide residue most frequently encountered in a glycan. The hydrosylate of lectin was prepared by treatment with trifluoroacetic acid, which resulted in the lectin mainly possessing the N-glycan consisting of 98.1 pmol Fuc, 342.1 pmol GlcN, 51.9 pmol Gal, 678.9 pmol Man, and 330.7 pmol Xyl. The present results demonstrate that ELLA and HPAEC-PAD are very effective methods for rapidly estimating the types and relative amounts of monosaccharides in intact glycoproteins.
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KEYWORD
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glycoprotein, enzyme-linked lectinsorbent assay, HPAEC-PAD, monosaccharide
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